关键词: 高血压肾病；单核苷酸多态性；HLA-DRB1基因

Abstract:

Abstract:Objective To explore the association between single nucleotide polymorphisms (SNPs) at rs2308765 and rs9269186 sites of HLA-DRB1 gene and hypertensive nephropathy, and to provide theroretical basis for study on genetic mechanism of hypertensive nephropathy. Methods Case-control study method was used and 97 cases were collected in the study. PCR-LDR technique was used to detect the SNPs of HLA-DRB1 in 45 patients with hypertensive nephropathy and 52 controls. The goodness-of-fit χ2test was used for Hardy-Weinberg equilibrium test in case and control groups. The differences of genotypic frequency and allelic frequency between case and control groups were test by χ2 test.Results There were G/T polymorphisms in  rs2308765 sites,two genotypes  G/G and G/T were found.The differences of genotype and allele distributions in rs2308765 between case and control were not significant (P>0.05). There were C/G polymorphisms in rs9269186 sites,three genotypes  C/C G/G and C/G were found.The differences of genotype and allele distributions in rs9269186 between case and control groups  were significant (P<0.05). Conclusion The correlation between SNPs at rs9269186 and hypertensive nephropathy is found, but not rs2308765.

Key words: hypertensive nephropathy； single nucleotide polymorphisms；HLA-DRB1 gene