关键词: 胶质瘤浸润性；金属蛋白酶；血管内皮生长因子及受体；多种肿瘤抑制基因1

Abstract:

Objective To detect the expressions of the matrix metalloproteinase 2(MMP-2) matrix metalloproteinase 9(MMP-9) vascular endothelial growth factor(VEGF）,Flms like tyrosine kinase (Flk-1) and multiple tumor suppressor 1 (P16) in human glioma invasive tissues,and to  elaborate the correlation of the MMP-2,MMP-9,VEGF,Flk-1 and P16 with the glioma invasion.Methods The expressions of MMP-2,MMP-9,VEGF,Flk-1 and P16 were detected through in situ hybridization and immunohistochemistry in 33  cases of glioma invasive tissues.Results
 The positive rates of MMP-2 and MMP-9 in Ⅰ-Ⅱ　grade and Ⅲ-Ⅳgrade glioma invasive tissues were 20.00%,61.11％ and 13.33％,55.56%. The positive rates of VEGF and Flk-1 were 26.67％,66.67％and 20.00％,7
2.22％(P<0.01),respecttively；the P16 deletion frequencies were 13.33％ and 77.78％,there was significant difference(P<0.01).Conclusion The necessary condition in occurrence of glioma invision is the basilar membrane degraded by the matrix metalloproteinases and the proliferative activity of vascular endothelial cells.
 The descended expression rate for P16 reflects the biological behavior of glioma invasive tissue.

Key words: glioma invasion；matrix metalloproteinases；vascular endothelial growth factor；flms like tyrosine kinase;multiple tumor suppressor 1