关键词:  帕金森病；酪氨酸羟化酶；诱导型一氧化氮合酶；白细胞介素-6；罗格列酮

Abstract:

Objective To investigate the regulation of rosiglitazone on iNOS and IL-6 in substantia nigra of the mice with Parkinson’s disease（PD）,and to clarify the mechanism. Methods 45 male mice were randomly divided into control group(n=15),model group(n=15) and rosiglitazone group(n=15). The behavioral changes of the mice in various  groups were observed by pole climbing experiment;immunohistochemistry and Western blotting methods were used to observe  the  expressions of iNOS,IL-6 and TH in substantia nigra of the mice. Results The model mice exhibited typical PD-like behaviors,such as tremor,gait retardation; compared with control group,the time of T-turn and T-LA in model group was prolonged,the differences between two groups were significant（P<0.05）; Compared with control group,TH-positive neurons lost obviously,the numbers of iNOS,IL-6 and TH immunoreactive cells and the expression levels in substantia nigra  of the midbrain were increased markedly,and the differences were statistically significant（P<0.05）.After treated with rosiglitazone,the  changes mentioned above were reduced significantly;there were significant differences of the numbers of iNOS,IL-6 and TH positive cells and their protein expression levels compared with model group（P<0.05）. Conclusion Rosiglitazone may protect the dopaminergic neurons by  inhibiting the expressions of iNOS and IL-6.

Key words: Parkinson’s disease, tyrosine hydr
oxylase, inducible nitric oxide synthase, interleukin-6, rosiglitazone