吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (06): 1243-1247.doi: 10.13481/j.1671-587x.20190609

• 基础研究 • 上一篇    下一篇

原花青素B1对LPS诱导小鼠巨噬细胞RAW264.7损伤的保护作用及其机制

张宸豪1, 李瑶1, 李正祎1, 骆晓峰2   

  1. 1. 吉林医药学院检验学院病原生物学教研室, 吉林 吉林 132013;
    2. 吉林医药学院基础医学院生理学教研室, 吉林 吉林 132013
  • 收稿日期:2019-08-24 出版日期:2019-12-05 发布日期:2019-12-05
  • 通讯作者: 骆晓峰,副教授(Tel:0432-64560323,E-mail:zhangchenhao0618@163.com) E-mail:zhangchenhao0618@163.com
  • 作者简介:张宸豪(1972-),男,吉林省吉林市人,讲师,医学博士,主要从事感染免疫相关疾病方面的研究。
  • 基金资助:
    国家自然科学基金资助课题(82102953);吉林省科技厅科研项目资助课题(20170311058YY);吉林省卫生厅科研项目资助课题(2013Z091)

Protective effect of procyanidine B1 on LPS-induced injury of mouse macrophages RAW264.7 and its mechanism

ZHANG Chenhao1, LI Yao1, LI Zhengyi1, LUO Xiaofeng2   

  1. 1. Department of Pathogen Biology, College of Medical Laboratory, Jilin Medical College, Jilin 132013, China;
    2. Department of Physiology, College of Basic Medical Sciences, Jilin Medical University, Jilin 132013, China
  • Received:2019-08-24 Online:2019-12-05 Published:2019-12-05

摘要: 目的:观察原花青素B1(PB1)对脂多糖(LPS)诱导的小鼠巨噬细胞RAW264.7损伤的保护作用,探讨其可能的作用机制。方法:体外培养的处于对数生长期的小鼠巨噬细胞RAW264.7分为对照组(细胞不进行处理)、LPS组(细胞给予2 mg·L-1LPS)、PB1组(细胞给予10 μmol·L-1 PB1)和PB1+LPS组(细胞给予2 mg·L-1LPS+10 μmol·L-1 PB1)。显微镜下观察各组细胞形态表现,流式细胞术检测各组细胞中活性氧(ROS)水平、细胞凋亡率和细胞表面膜分子CD16/32、CD40、CD86及Toll样受体4(TLR4)表达水平。结果:与对照组比较,LPS组细胞皱缩变圆,但PB1组和PB1+LPS组细胞形态表现变化不明显。与对照组比较,LPS组细胞中ROS水平明显升高(P<0.05);与LPS组比较,PB1+LPS组细胞中ROS水平降低(P<0.05)。与对照组比较,LPS组细胞凋亡率升高(P<0.05);与LPS组比较,PB1+LPS组细胞凋亡率降低(P<0.05)。与对照组比较,LPS组细胞表面膜分子CD16/32、CD40、CD86和TLR4表达水平升高(P<0.05);与LPS组比较,PB1+LPS组细胞表面膜分子CD16/32、CD40、CD86和TLR4表达水平降低(P<0.05)。结论:LPS通过诱导细胞中ROS水平升高引起细胞损伤,PB1通过降低ROS水平,下调细胞表面膜分子CD16/32、CD40、CD86和TLR4表达对细胞发挥保护作用。

关键词: 原花青素B1, 巨噬细胞, 脂多糖, 损伤, 细胞凋亡

Abstract: Objective: To observe the protective effect of procyanidin B1 (PB1) on the lipopolysaccharide (LPS)-induced injury of macrophages RAW264.7, and to explore its possible mechanism. Methods: The macrophages RAW264.7 in logarithmic phase cultured in vitro were divided into control group (without treatment), LPS group (treated with 2 mg·L-1 LPS), PB1 group (treated with 10 μmol·L-1 PB1) and PB1 + LPS group (treated with 2 mg·L-1 LPS and 10 μmol·L-1 PB1). The morphology of cells was observed under microscope, the levels of reactive oxygen species (ROS) in the cells,the apoptotic rates of the cells and the expression levels of CD16/32, CD40, CD86, and Toll-like receptor 4(TLR4) on cell surface of the cells in various groups were detected by flow cytometry. Results: Compared with control group, the cells in LPS group were shrunk and round,but the morphological changes of the cells in PB1 group and PB1 + LPS group were not significant. Compared with control group, the ROS level in the cells in LPS group was significantly increased (P<0.05); compared with LPS group, the ROS level in the cells in PB1 + LPS group was significantly decreased (P<0.05). Compared with control group, the apoptotic rate of the cells in LPS group was decreased (P<0.05); compared with LPS group, the apoptotic rate of the cells in PB1 + LPS group was increased(P<0.05). Compared with control group, the expression levels of cell surface molecules CD16/32, CD40, CD86, and TLR4 in the cells in LPS group were increased (P<0.05); compared with LPS group, the expression levels of cell surface molecules CD16/32, CD40, CD86, and TLR4 in the cells in PB1 + LPS group were decreased (P<0.05). Conclusion: LPS can induce the cell injury by increasing the ROS level, and PB1 can protect the cells by decreasing the ROS level and down-regulating the expression levels of cell surface molecules CD16/32, CD40, CD86, and TLR4.

Key words: procyanidin B1, macrophages, lipopolysaccharide, damage, apoptosis

中图分类号: 

  • R392.28