硒蛋白P基因多态性与结直肠癌遗传易感性的关联性分析

doi:10.13481/j.1671-587x.20150518

Abstract

Abstract:

Objective To investigate the correlation between the polymorphisms of rs7579 site at selenoprotein P(Sepp1) gene and the genetic susceptibility of colorectal cancer,and to provide the theoretical basis for studying the genetic mechanism of colorectal cancer. Methods A case-control study was used in this research.The genotypic and allelic frequencies of Sepp1 gene were detected both in 130 patients with colorectal cancer and 153 healthy people by tetra-primer amplification refractory mutation system-polymerase chain reaction(tetra-primer ARMS-PCR)method,which was confirmed by gene sequencing analysis.The association between genotypic and allelic frequencies of rs7579 site at Sepp1 gene and genetic susceptibility of colorectal cancer was analyzed,then the association between the genotypes of rs7579 site at Sepp1 gene and genetic susceptibility of colorectal cancer in the patients with colorectal cancer with different clinical features was also analyzed. Results The distribution of polymorphism of rs7579 site at Sepp1 gene did not deviate from Hardy-Weinberg equilibrium in both case and control groups(P>0.05).There were no significant differences of the genotypic and allelic frequencies of rs7579 site at Sepp1 gene site between case group and control group(P>0.05).The stratification analysis results showed that there was significant difference of the genotypic frequency of rs7579 site between case group and control group in the patients more than 55 years old(χ²=3.228,P=0.050),and the genotypic frequency of AA of the patients in case group was significantly lower than that in control group(χ²=3.228,P=0.050,OR=0.566,95%CI:0.320-0.999). Conclusion The polymorphism of rs7579 site at Sepp1 gene is irrelevant to the pathogeny of colorectal cancer.Allele A may be one of the protective factors that reduce the morbidity of colorectal cancer among Chinese people older than 55 years old.

Key words: selenoprotein P, colorectal neoplasms, genetic susceptibility, single nucleotide polymorphism

CLC Number:

R735.3

ZHANG Long, ZHU Yan, WANG Xiaochun, ZHANG Junfeng, HU Ruxin, LI Yufei, LI Chunbo. Analysis on correlation between genetic susceptibility of colorectal cancer and polymorphisms of selenoprotein P[J].Journal of Jilin University Medicine Edition, 2015, 41(05): 980-985.

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Analysis on correlation between genetic susceptibility of colorectal cancer and polymorphisms of selenoprotein P

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