Journal of Jilin University Medicine Edition ›› 2016, Vol. 42 ›› Issue (04): 716-720.doi: 10.13481/j.1671-587x.20160416

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Influence of antophagy in radiation sensitivities of oral squamous cell carcinoma CAL-27 and KB cells and its mechanisms

XU Zhaonan1,2, BI Ye1,2, WANG Xi3, ZHANG Zebing1,2, WANG Shuyu1,2, JIANG Siwen3, JIA Jie4   

  1. 1. Department of Pathology, Stomatology Hospital, Jilin University, Changchun 130021, China;
    2. Jinlin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Jilin University, Changchun 130021, China;
    3. Department of Radiotherapy, Second Hospital, Jilin University, Changchun 130041, China;
    4. Department of Electrical Diagnosis, China-Japan Union Hospital, Jilin University, Changchun 130033, China
  • Received:2016-01-24 Published:2016-07-20

Abstract:

Objective: To use autophagy inhibitors combined with radiation to treat the oral squamous cell carcinoma CAL -27 and KB cells,and to explore the influence of autophagy in the oral cancer radiation sensitivity and its mechanisms. Methods: The human oral squamous cell carcinoma CAL-27 and KB cells were divided into control group,CQ group,3-MA group,IR group,CQ+IR group, and 3-MA+IR group. The survival rate was detected by MTT method and the autophagy of CAL-27 cells was observed by immunofluorescence method and laser scanning confocal microscope.The expression levels of LC3 and beclin-1 were detected by Western blotting method.The apoptotic rate was determined by flow cytometry with Annexin Ⅴ/PI doulde staining. Results: Compared with IR group,the survival rates in 3-MA+IR and CQ+IR groups were signifcantly decreased (P < 0.05).The autophagy levels of cells in IR group were significantly higher than those in control group,CQ group,3-MA group,CQ+IR group, and 3-MA+IR group(P < 0.05).The expression levels of LC3 and beclin-1 proteins in IR group were significantly higher than those in control group,CQ+IR group,and 3-MA+IR group(P < 0.05).The apoptotic rates in IR,3-MA+IR, and CQ+IR groups were markedly higher than those in control group.Compared with IR group,the apoptotic rates in CQ+IR and 3-MA+IR groups were significantly increased (P < 0.05). Conclusion: Radiotherapy can induce the increase of autophagy level of oral squamous cell carcinoma cells. Inhibiors of autophagy can increase the radio-sensitivity of oral squamous cell carcinoma cells by inhibiting the proliferation and inducing the apoptosis.

Key words: autophagy, radiotherapy, chloroquine, 3-methyladenine, mouth neoplasms

CLC Number: 

  • R739.8