CD14和IL-8基因多态性与新生儿坏死性小肠结肠炎易感性的关联性分析

doi:10.13481/j.1671-587x.20160523

Abstract

Abstract:

Objective: To investigate the relationship between the gene polymorphism cluster of differentiation 14 (CD14)-159C/T (rs2569190), and interleukin-8 (IL-8)-251A/T (rs4073) and the susceptibility of necrotizing enterocolitis (NEC), to clarify the influencing factors of susceptibility of NEC and to provide genetics theory basis for the research on the pathogenesis of NEC. Methods: Total 28 newborns with NEC and 41 newborns without NEC were selected. The amplification of peripheral blood DNA was conducted by PCR. The genotypic and allelic frequencies of CD14-159C/T and IL-8-251A/T of the patients were detected by Sanger DNA sequencing method. The relationship between them and the susceptibility of NEC was studied. Results: The distribution of genotypic frequencies of CD14-159C/T and IL-8-251A/T was consistent with Hardy-Weinberg equilibrium(P>0.05). There were no significant differences of the allelic and genotypic frequencies of CD14-159C/T, or genotypic frequencies of IL-8-251A/T between two groups(P>0.05). While in NEC group,the T allelic frequency of IL-8-251A/T site was higher than that in control group(χ²=4.184,P=0.041, OR=2.14,95%CI:1.03-4.46). Conclusion: The polymorphism of CD14-159C/T is irrelevant to the pathogeny of NEC, but T allelic frequency of IL-8-251A/T site might be related to the susceptibility of NEC. So T allele in IL-8-251A/T may be one of the danger factors of NEC.

Key words: necrotizing enterocolitis, cluster of differentiation 14, interleukin-8, single nucleotide polymorphism

CLC Number:

R722.19

TIAN Jiayi, ZHU Tong, WANG Jian, FANG Mingli, YAN Chaoying. Analysis on association between polymorphism of CD14 and IL-8 gene and susceptibilityof necrotizing enterocolitis[J].Journal of Jilin University Medicine Edition, 2016, 42(05): 958-962.

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Analysis on association between polymorphism of CD14 and IL-8 gene and susceptibilityof necrotizing enterocolitis

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