Journal of Jilin University(Medicine Edition) ›› 2021, Vol. 47 ›› Issue (4): 857-864.doi: 10.13481/j.1671-587X.20210406
• Research in basic medicine • Previous Articles Next Articles
Xining LI1,Wei WENG2,Zheyuan SHEN2,Xiaojie DOU2,Jikang MIN2()
Received:
2020-11-08
Online:
2021-07-28
Published:
2021-07-22
Contact:
Jikang MIN
E-mail:2504074093@qq.com
CLC Number:
Xining LI,Wei WENG,Zheyuan SHEN,Xiaojie DOU,Jikang MIN. Therapeutic effect of estradiol combined with 1,25-dihydroxyvitamin D3 on postmenopausal osteoporosis in rats[J].Journal of Jilin University(Medicine Edition), 2021, 47(4): 857-864.
Tab.1
Primer sequences of ALP,OC, OPG and RANKL"
Primer | Sequence | Size(bp) | |
---|---|---|---|
ALP | F | 5'-TGCCCTGAAACTCCAAAAGC-3' | 215 |
R | 5'-CTTCACGCCACACAAGTAGG-3' | ||
OC | F | 5'-CGCTACTGTGTCTTGGCATC-3' | 168 |
R | 5'-GGTTCATGCTGCTTAGTCGG-3' | ||
OPG | F | 5'-AGGGTTTCATTCCAGGATCGAGC-3' | 186 |
R | 5'-CGGGAGAACAGGGTATGA-3' | ||
RANKL | F | 5'-CGGGAGAACAGGGTATGA-3' | 209 |
R | 5'-CAGAGACAGCCAGGAGAA-3' |
Tab.2
Levels of osteogenesis- and osteoclast-related indexes in serum of rats in various groups"
Group | ALP [λB/(U·L-1)] | OC [ρB/(μg·L-1)] | OPG [ρB/(μg·L-1)] | PINP [ρB/(μg·L -1)] | TRACP [λB/(U·L-1)] | RANKL [ρB/(μg·L-1)] | OPG/RANKL |
---|---|---|---|---|---|---|---|
Control | 88.865±2.895 | 1.179±0.177 | 8.657±1.239 | 12.290±0.801 | 15.236±0.960 | 1.552±0.191 | 5.663±1.273 |
Model | 127.161±8.473* | 4.909±0.643* | 14.093±1.329* | 19.803±1.554* | 31.363±2.441* | 3.488±0.494* | 4.133±0.899 |
Estradiol | 90.680±12.223△ | 2.919±0.253△ | 9.916±1.080△ | 12.610±1.724△ | 17.828±1.683△ | 1.730±0.580△ | 6.005±2.044 |
Estradiol combined with 1,25(OH)2D3 | 96.316±13.252△ | 4.157±0.216# | 14.526±1.610# | 11.372±1.769△ | 8.063±2.054△# | 0.819±0.184△# | 18.226±3.414△# |
Tab.3
Femoral BMD and biomechanical indexes of rats in various groups"
Group | BMD (mg·cm-2) | Elastic modulus (P/Mpa) | Stiffness (N·mm-1) | Maximum stress (N·mm-2) | Maximum lord (F/N) |
---|---|---|---|---|---|
Control | 123.645±10.167 | 635.809±64.746 | 872.072±38.823 | 21.131±1.535 | 103.778±4.979 |
Model | 67.092±5.110* | 337.594±60.210* | 612.921±106.258* | 9.138±1.723* | 48.453±7.721* |
Estradiol | 97.870±5.557△ | 493.003±65.654△ | 813.682±22.937△ | 15.624±3.054△ | 96.192±4.170△ |
Estradiol combined with 1,25(OH)2D3 | 132.234±11.911△# | 670.116±87.066△# | 856.421±42.933△ | 20.524±1.707△# | 99.825±8.595△ |
Tab.4
Expression levels of mRNA of osteogenesis-and osteoclast-related indexes in bone tissue of rats in various groups (n=3,x±s)"
Group | ALP | OC | OPG | RANKL |
---|---|---|---|---|
Control | 1 | 1 | 1 | 1 |
Model | 1.551±0.173* | 2.806±0.647* | 3.148±0.061* | 2.598±0.407* |
Estradiol | 1.041±0.102△ | 1.543±0.371△ | 1.340±0.307△ | 1.085±0.160△ |
Estradiol combined with 1,25(OH)2D3 | 1.060±0.279△ | 1.622±0.615△ | 3.479±0.618# | 0.390±0.223△# |
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