益生菌对卵巢摘除致雌激素缺乏小鼠牙周组织中IL-17表达的影响及其意义

doi:10.13481/j.1671-587x.20200411

Abstract

Abstract: Objective: To explore the effect of probiotic feeding on the interleukin-17 (IL-17) expression in periodontal tissue of the estrogen-deficient mice, and to elucidate the possible mechanism of the correlation between estrogen deficiency and the periodontitis susceptibility. Methods: A total of 15 female C57BL/J6 mice(8-week-old) were randomly divided into sham operation group, ovariectomy group and probiotics group (probiotics feeding after ovariectomy) (n=5). The bone mineral densities(BMD) of the mice in various groups before and after experiment were measured by mean bone density measurement technique and the IL-17 mRNA expression levels in bone marrow cells and gingiva tissus of the mice were determined by RT-qPCR. The percentages of Th17 cells in CD4⁺TCRβ⁺ B cells in bone marrow of the mice were measured by flow cytometry. Results: Compared with sham operation group, the BMD of femur of the mice in OVX group was significantly decreased (P<0.01), the expression levels of IL-17 mRNA in bone marrow cells and gingiva tissue were increased (P<0.01), and the percentage of CD4⁺TCRβ⁺Th17 cells in the bone marrow was increased(P<0.05).Compared with OVX group, the BMD of femur of the mice in probiotics group was significantly increased (P<0.01), the expression levels of IL-17 mRNA in bone marrow cells and gingiva tissue were decreased (P<0.01), and the percentage of CD4⁺TCRβ⁺Th17 cells in the bone marrow was decreased(P<0.05).Compared with sham operation group, the BMD of femur, the expression levels of IL-17 mRNA in bone marrow cells and gingiva tissue, and the percentage of CD4⁺TCRβ⁺Th17 cells in the bone marrow in probiotics group had no significant differences (P>0.05). Conclusion: Probiotics feeding can inhibit the upregulation of IL-17 mRNA level of the mice induced by estrogen deficiency, and has the preventive effect on the estrogen deficiency-induced periodontitis.

Key words: estrogen deficiency, probiotic, interleukin-17, T helper cell 17, periodontitis

CLC Number:

R781.4

HUANG Jiangyong, LI Chanxiu, ZHENG Zhichao, WANG Hongjuan, GUO Lyuhua, WU Zhe, LUO Tao. Effect of probiotics on expression of IL-17 in periodontal tissue of estrogen-deficient mice induced by ovariectomy and its significance[J].Journal of Jilin University(Medicine Edition), 2020, 46(04): 733-738.

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Effect of probiotics on expression of IL-17 in periodontal tissue of estrogen-deficient mice induced by ovariectomy and its significance

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