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Extracting Lilium Brownii Polysaccharide by Combined-enzymes and Its Antioxidative Activity in Vitro
TENG Li-rong, MENG Qing-fan, LIU Pei-yuan, QIAO Xiao-hang, WANG Zhao-fu , HE Qiu-hua, HONG Shui-sheng, CHEN Jia, LIU Lan-ying
J4. 2003, 41 (04):
538-542.
Two methods of extracting Lilium brownii polysaccharide, hot-water method and combined-enzyme method, were studied and the optimum conditions of t he two methods were found. The yield of the polysaccharides extracted by combine d enzymes was 2.8 times that of polysaccharides extracted by hot-water. It was also found that the antioxidative activity of the polysaccharides, such as i nhibiting effects on O-2·, ·OH, the hemolysis of human R BC induced by H2O2 and the formation of MDA, extract ed by combined-enzymes were much greater than that of those extracted by hot-wa ter. After extracted by the two methods, the polysaccharides were separated by s ephedex G-75 gel filtration chromotography and three fractions were eluted out. LBP-3, whose molecular weight was about 13 400 and had the highest antioxida tive activity of the three fractions, was found to exist in polysaccharides extr ated by combined enzymes much more than that to exist in those extracted by hot-water.
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