吉林大学学报(医学版)

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水飞蓟宾对人膀胱癌细胞系T24和5637的增殖抑制及凋亡诱导作用

迟庆龙,王艳波,王春喜   

  1. (吉林大学第一医院泌尿外科,吉林 长春 130021)
  • 收稿日期:2013-08-22 出版日期:2014-03-28 发布日期:2014-03-28
  • 通讯作者: 王春喜 E-mail:chunxi_wang@126.com
  • 作者简介:迟庆龙(1988-),男,吉林省吉林市人,在读医学硕士,主要从事泌尿外科肿瘤发病机制与治疗研究。
  • 基金资助:

    吉林省科技厅自然科学基金资助课题(201115047)

Inhibitory  effects of silibinin on proliferation  and apoptosis induction of human bladder cancer T24 and 5637 cell lines

 CHI Qing-long,WANG Yan-bo,WANG Chun-xi   

  1. (Department of Urology,First Hospital,Jilin University,Changchun 130021,China)
  • Received:2013-08-22 Online:2014-03-28 Published:2014-03-28

摘要:

目的:探讨水飞蓟宾(SB)对人膀胱癌细胞系T24和5637增殖及凋亡的影响,初步阐明其可能的机制。方法:培养人膀胱癌细胞系5637和T24,取处于对数生长期的5637和T24细胞分为对照组(0 μmol•L-1 SB)及25、50、100、200和400  μmol•L-1 SB 组,MTT法检测SB对人膀胱癌细胞系T24和5637的增殖抑制作用;采用倒置显微镜观察不同浓度SB作用不同时间对人膀胱癌细胞系T24和5637的侵袭抑制能力;DAPI染色法观察给药后细胞形态;流式细胞术检测并评估SB诱导肿瘤细胞凋亡的能力。结果:MTT法检测,随着SB浓度的增加和作用时间延长,不同浓度SB组T24和5637细胞存活率显著低于对照组;不同浓度SB处理后,各组细胞的迁移明显受到抑制;DAPI染色及流式细胞术检测,不同浓度SB组T24和5637细胞凋亡率显著高于对照组(P<0.05)。结论:SB通过诱导人膀胱癌细胞系T24和5637凋亡从而抑制细胞侵袭及增殖。

关键词: 水飞蓟宾, 膀胱肿瘤, 细胞增殖, 细胞凋亡

Abstract:

o determine the effects of silibinin(SB) on the proliferation  and apoptosis of human bladder cancer T24 and 5637 cell lines,and to clarify the possible mechanism.
Methods Human bladder cancer 5637 and T24 cell lines  were cultured in cell media and the  5637 and T24 cells at logarithmic growth phase were divided into control group(0 μmol•L-1  SB) and 25,50,100,200,and 400  μmol•L-1  SB groups.The inhibitory effects of SB on the proliferation  of T24 and 5637 cells were  detected by  MTT assay;inverted phase contrast microscope was used to observe the invasion inhibition ability of T24 and 5637 cell lines after treated with different contentations of SB;the morphological changes of T24 and 5637 cells after treatment were observed by DAPI staining;the apoptosis   was detected and evaluated by flow cytometry.Results The  MTT results showed that  the  viability rates of  T24 and 5637 cell lines were decreased    in different concentrations of SB groups in a time- and concentration-dependent  manner compared with  control group.After treated with different concentrations of SB,the migration of T24 and 5637 cell lines was significantly inhibited.The DAPI staining and flow cytometry results revealed that the apoptotic rates of T24 and 5637 cells  in different concentrations of SB groups were higher than those in control group(P<0.05).Conclusion SB can  inhibit the proliferation and invasion of human bladder cancer T24 and 5637 cell lines  through inducing the apoptosis.

Key words: silibinin, bladder neoplasms, cell proliferation, apoptosis

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