银丹心脑通软胶囊对大鼠急性心肌梗死后心肌组织中骨桥蛋白表达的影响及其心肌保护作用机制

doi:10.13481/j.1671-587x.20140320

吉林大学学报(医学版)

银丹心脑通软胶囊对大鼠急性心肌梗死后心肌组织中骨桥蛋白表达的影响及其心肌保护作用机制

王树东¹,丁丽娟¹,耿嘉男²，李特¹,刘全¹

1.吉林大学第一医院心血管疾病诊疗中心，吉林长春 130021；2.吉林大学药学院实验药理与毒理学教研室，吉林长春130021

收稿日期:2013-08-29 出版日期:2014-05-28 发布日期:2014-06-05
通讯作者: 刘全（Tel:0431-88789485，E-mail:quanliu888@163.com） E-mail:quanliu888@163.com
作者简介:王树东（1979-），男，吉林省榆树市人，主治医师，医学硕士，主要从事心血管疾病诊治方面的研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题 (200765122)

Effects of Yindan Xinnaotong Capsule on OPN expression in myocardium tissue of rats after acute myocardial infarction and its prevention mechanism on myocardium

WANG Shu-dong¹,DING Li-juan¹,GENG Jia-nan²,LI Te¹,LIU Quan¹

1. Cadiovascular Disease Center,First Hospital,Jilin University,Changchun 130041,China; 2. Department of Experimental Phyarmacology and Toxicology,School of Pharmacy,Jilin University,Changchun 130021,China

Received:2013-08-29 Online:2014-05-28 Published:2014-06-05

摘要/Abstract

摘要：

目的：探讨银丹心脑通软胶囊对大鼠急性心肌梗死（AMI）后心肌组织中骨桥蛋白（OPN）表达的影响，阐明银丹心脑通软胶囊改善大鼠AMI的作用机制。方法：将90只Wistar大鼠结扎冠脉前降支建立AMI模型，术后存活动物随机分为模型组、银丹心脑通软胶囊小剂量组（银丹心脑通软胶囊0.8 g/kg/d）、银丹心脑通软胶囊大剂量组（银丹心脑通软胶囊1.6 g/kg/d ）、阳性药卡托普利组（卡托普利5 g/kg/d），每组12只，同时设假手术组(只穿线不结扎)(n=10)，连续给药4周。HE和Masson染色观察各组大鼠心肌组织学表现；脱氧核苷酸末端转移酶介导的缺口末端标记法（TUNEL）原位检测凋亡细胞的DNA碎片，计算凋亡指数（AI）；RT-PCR法检测非梗死区心肌中OPN mRNA表达水平。结果：与假手术组比较，模型组大鼠AI明显升高(P<0.05)；与模型组比较，各给药组大鼠AI明显降低（P<0.01）；与卡托普利组比较，银丹心脑通大剂量组大鼠AI明显降低，差异有统计学意义(P<0.05)，银丹心脑通小剂量组大鼠AI无明显改变(P>0.05)。RT-PCR检测，与假手术组比较，模型组大鼠左心室非梗死区心肌组织中OPN mRNA表达水平明显升高(P<0.01)；与模型组比较，各给药组大鼠左心室非梗死区心肌组织中OPN mRNA表达水平明显降低（P<0.01）；与卡托普利组比较，银丹心脑通大剂量组大鼠左心室非梗死区心肌组织中OPN　mRNA表达水平明显降低(P<0.05)，而银丹心脑通小剂量组OPN　mRNA表达水平无明显改变（P>0.05）。结论：银丹心脑通软胶囊能够显著改善心肌梗死后大鼠心肌组织细胞凋亡，降低左心室非梗死区OPN mRNA表达水平，提示银丹心脑通软胶囊对大鼠AMI的心肌保护作用可能与降低OPN mRNA表达有关。

关键词: 银丹心脑通软胶囊, 急性心肌梗死, 细胞凋亡, 骨桥蛋白

Abstract:

Abstract:Objective To investigate the effects of Yindan Xinnaotong Capsule on the osteoponin（OPN）expression in myocardium tissue of the rats after acute myocardial infarction(AMI) and to clarify the mechanism of Yindan Xinnaotong Capsule in alienating rat AMI.Methods 90 Wistar rats were used to establish AMI models by ligating of the left anterior descending coronary artery.The AMI model rats were randomly divided into　AMI model group,high dose of Yindan (16 g/kg/d) group,low dose of Yindan(0.08 g/kg/d) group,positive drug control captopril(5 mg/kg/d) group(n=12)；at the same time sham operation group(n=10) was set up,the rats in sham operation group was treated with wearing without ligation.All the rats were administrated for 4 weeks,then the myocardium tissue samples were obtained.The histological changes of myocardium tissue were observed by HE and Masson staining;the DNA fragments of apoptotic cells were detected by TUNEL staining and the apoptotic index(AI)was calculated.The expression of OPN mRNA in non-infarction area was measured by RT-PCR.Results Compared with sham operation group,the AI of the rats in model group was significantly increased(P<0.05)； compared with model group,the AI of the rats in Yindan groups and captopril group were markedly decreased(P<0.01);compared with captopril group,the AI of the rats in high dose of Yindan group was significantly decreased(P<0.05).Compared with sham operation group,the expression level of OPN mRNA in non-infarction area of the rats in model group was significantly increased(P<0.01);compared with model group,the expression levels of OPN mRNA in non-infarction area of the rats in Yindan groups and captopril group were also decreased significantly(P<0.01);compared with captopril group,the expression level of OPN mRNA non-infarction area of the rats in high dose of Yindan group was significantly decreased(P<0.05).Conclusion Yindan Xinnaotong Capsule could obviously alleviate the apoptosis of myocardium tissue of the rats after acute myocardial infarction and decrease the expression of OPN mRNA in non-infarction area of left ventricle,which indicates that Yindan Xinnaotong Capsule may protect myocardium tissue through decreasing the OPN mRNA expression.

Key words: Yindan Xinnaotong capsule, acute myocardial infarction, apoptosis, osteopontin

中图分类号:

R541.4

王树东,丁丽娟,耿嘉男，李特,刘全. 银丹心脑通软胶囊对大鼠急性心肌梗死后心肌组织中骨桥蛋白表达的影响及其心肌保护作用机制[J]. 吉林大学学报(医学版), 2014, 40(03): 564-568.

WANG Shu-dong,DING Li-juan,GENG Jia-nan,LI Te,LIU Quan. Effects of Yindan Xinnaotong Capsule on OPN expression in myocardium tissue of rats after acute myocardial infarction and its prevention mechanism on myocardium[J]. Journal of Jilin University Medicine Edition, 2014, 40(03): 564-568.

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银丹心脑通软胶囊对大鼠急性心肌梗死后心肌组织中骨桥蛋白表达的影响及其心肌保护作用机制

Effects of Yindan Xinnaotong Capsule on OPN expression in myocardium tissue of rats after acute myocardial infarction and its prevention mechanism on myocardium

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