关键词: P53凋亡刺激蛋白, 结肠肿瘤, 免疫组织化学, 细胞凋亡

Abstract:

Abstract:Objective To investigate the expressions of apoptosis stimulating protein of P53 (ASPP) family in colorectal carcinoma tissue and to explore their relationship with the clinicopathological characteristics of colorectal carcinoma,and to clarify the effect of ASPP  family in the development of colorectal carcinoma.
Methods 45 cases of colorectal carcinoma tissue and 20　cases of healthy controls were selected.Among 45 cases of colorectal carcinoma tissue,there were
 11 cases of well differentiated colorectal carcinoma,21 cases of moderately differentiated colorectal carcinoma,and 13 cases of poorly differentiated color
ectal carcinoma;7 cases of T1 stage colorectal carcinoma,8 cases of T2 stage colorectal carcinoma,25 cases of T3 stage colorectal carcinoma,and 5 cases of T4
 stage colorectal carcinoma;19 cases of N1 stage with lymph node metastasis,26 cases of N0 stage without lymph node metatasis.The expressions of ASPP1,ASPP2,and iASPP in 45 cases of colorectal carcinoma tissue  and 20 cases of normal colon tissue were detected by immunohitochemistry SP method,and the correlations between the expressions of ASPP  family and the pathologic typing,infiltrative depth,and lymph node metastasis of colorectal carcinoma were analyzed.Results ①The immunohitochemical staining results showed that the ASPP  family members expressed in colorectal carcinoma tissue and normal colon tissue,and there were no significant differences in ASPP1 and ASPP2 positive rates between colorectal carcinoma tissue and normal colon tissue (P>0.05);the positive expression rate of iASPP in colon cancer tissue was higher than that in normal colon tissue (P<0.01).② The expression of ASPP1 in colon caner tissue had no correlation with the differentiation degree of tumor cells (rs =0.163,P>0.05);the expression of ASPP2 positive rate was decreased when the differentiation degree of tumor cells reduced,they had positive correlation (rs =0.454,P=0.002)；the expression of iASPP in colon cancer tissue had no correlation with the differentiation degree of tumor cells (rs =－0.171,P>0.05).③ The expression of ASPP1 in colon caner tissue had no correlation with the infiltrative depth of tumor (rs =－0.268,P>0.05);the expression of ASPP2 positive rate was decreased when the tumor infiltrative depth increased,they had negative correlation (rs=－0.348,P<0.05);the expression of iASPP in colonic carcinoma caner tissue had no correlation with the infiltrative depth of tumor (rs =0.231,P>0.05).④The expressions of ASPP1,ASPP2,and iASPP in colon caner tissue had no correlation with lymph node metastasis (rs =0.089,rs =0.044,rs =0.210,P>0.05).Conclusion The expression levels of iASPP in colon cancer and normal colon tissues are different,it may be useful for the diagnosis,differential diagnosis and evaluation in benign and malignant colorectal diseases.   The expression of iASPP is negatively correlated with the pathologic typing and neoplasm staging of colorectal carcinoma,it indicates that iASPP can be used as a indicator in judging the prognosis of colorectal carcinoma.

Key words: apoptosis stimulating protein of P53, colorectal neoplasms, immunohistochemistry, apoptosis