吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (06): 1379-1383.doi: 10.13481/j.1671-587x.20190631

• 基础研究 • 上一篇    下一篇

抑制TRIM24基因表达对乳腺癌MCF-7细胞增殖、凋亡、侵袭和迁移的抑制作用

杨庄青, 陆眉, 杨晓娟, 王常安, 邹洁雅   

  1. 昆明医科大学第三附属医院 云南省肿瘤医院乳腺二科, 云南 昆明 650118
  • 收稿日期:2018-12-27 出版日期:2019-12-05 发布日期:2019-12-05
  • 作者简介:杨庄青(1974-),女,苗族,湖南省凤凰县人,副教授,医学硕士,主要从事乳腺肿瘤和乳房整形方面的研究。
  • 基金资助:
    云南省自然科学基金资助课题(2017FZ239)

Inhibitory effect of inhibiting TRIM24 gene expression on proliferation, apopotosis,invasion and migration of breast cancer MCF-7 cells

YANG Zhuangqing, LU Mei, YANG Xiaojuan, WANG Chang'an, ZOU Jieya   

  1. Department of Breast Surgery, Third Affiliated Hospital, Kunming Medical University, Yunnan Cancer Hospital, Kunming 650118, China
  • Received:2018-12-27 Online:2019-12-05 Published:2019-12-05

摘要: 目的:探讨抑制TRIM24基因表达对乳腺癌MCF-7细胞增殖、凋亡、侵袭和迁移的影响,阐明TRIM24基因在乳腺癌发生发展中的作用。方法:以脂质体法转染TRIM24 siRNA和阴性对照NC-siRNA至MCF-7细胞作为si-TRIM24组和NC-siRNA组,以未转染的MCF-7细胞作为对照组。qRT-PCR和Western blotting法检测转染48 h后各组MCF-7细胞中TRIM24 mRNA和蛋白表达水平,MTT法检测各组MCF-7细胞增殖能力,流式细胞术检测各组MCF-7细胞凋亡率,Transwell实验检测各组MCF-7细胞侵袭和迁移数。结果:与对照组和NC-siRNA组比较,si-TRIM24组MCF-7细胞中TRIM24 mRNA和蛋白表达水平明显降低(P<0.05),MCF-7细胞增殖能力降低(P<0.05),MCF-7细胞凋亡率明显升高(P<0.05),MCF-7细胞侵袭和迁移数明显减少(P<0.05)。结论:抑制TRIM24基因表达能够明显抑制乳腺癌MCF-7细胞的增殖、侵袭和迁移,且能够诱导细胞凋亡。

关键词: TRIM24基因, 乳腺肿瘤, 细胞增殖, 细胞凋亡, 侵袭, 迁移

Abstract: Objective: To investigate the effect of inhibiting TRIM24 gene expression on the proliferation, apoptosis, invasion and migration of breast cancer MCF-7 cells, and to elucidate the role of TRIM24 gene in the occurrence and development of breast cancer. Methods: The TRIM24 siRNA and negative control NC-siRNA were transfected into the MCF-7 cells by liposome method, and used as si-TRIM24 group and NC-siRNA group, respectively. The untransfected MCF-7 cells were used as control group. qRT-PCR and Western blotting methods were used to detect the expression levels of TRIM24 mRNA and protein in the MCF-7 cells in various groups after transfected for 48 h,MTT assay was performed to detect the proliferation activities of the MCF-7 cells in various groups,flow cytometry was used to determine the apoptotic rates of the MCF-7 cells in various groups,and Transwell assay was used to measure the number of invasion and migration cells in various groups. Results: Compared with control group and NC-siRNA group, the expression levels of and protein in the MCF-7 cells were significantly decreased (P<0.05), and the proliferation activity of the MCF-7 cells was decreased (P<0.05), the apoptotic rate of the MCF-7 cells was significantly increased (P<0.05), and the number of invasion and migration MCF-7 cells was significantly decreased (P<0.05). Conclusion: Inhibition of the expression of TRIM24 gene can significantly inhibit the proliferation, invasion and migration of breast cancer MCF-7 cells, and induce the apoptosis.

Key words: TRIM24 gene, breast neoplasms, cell proliferation, apoptosis, invasion, migration

中图分类号: 

  • R737.9