哮喘小鼠肺脏组织肾素-血管紧张素系统相关组分表达
及AT1R拮抗剂对其影响

J4 ›› 2011, Vol. 37 ›› Issue (6): 1065-1069.

哮喘小鼠肺脏组织肾素-血管紧张素系统相关组分表达
及AT₁R拮抗剂对其影响

孙璐瑶|刘颖|于振香

吉林大学第一医院呼吸科|吉林长春130021

收稿日期:2011-05-18 出版日期:2011-11-28 发布日期:2011-11-28
通讯作者: 于振香（Tel：0431-88782305,E-mail：yuzhenxiang2005@sina.com） E-mail:yuzhenxiang2005@sina.com
作者简介:孙璐瑶（1987-）|女|吉林省长春市人|在读医学硕士|主要从事支气管哮喘发病机制的研究。
基金资助:
吉林省科技厅科研基金资助课题(20110454)

Expressions of component of renin - angiotensin system in lung tissue of asthmatic mice and effect of AT1 receptor blocker

SUN Lu-yao,LIU Ying,YU Zhen-xiang

Department of Respiratory Medicine|First Hospital| Jilin University| Changchun 130021|China

Received:2011-05-18 Online:2011-11-28 Published:2011-11-28

摘要/Abstract

摘要：

目的：探讨肾素-血管紧张素系统（RAS）与哮喘发生的关系，为哮喘发病机制的研究和治疗提供理论依据。方法：复制小鼠哮喘模型，小鼠分为对照组、模型组、坎地沙坦低剂量组及高剂量组，采集小鼠血清，测量血清中血管紧张素Ⅱ (AngⅡ)与血管紧张素Ⅰ(AngⅠ)的含量；通过Western blotting和RT-PCR观察RAS中血管紧张素原(AGT)、血管紧张素转换酶(ACE)、血管紧张素Ⅱ 1型受体（AT₁R）和2型受体（AT₂R）在各组小鼠肺组织中的表达。结果：模型组小鼠血清AngⅡ含量较对照组增高（P<0.05），坎地沙坦组与模型组比较无明显变化（P>0.05）。各组小鼠血清中AngⅠ含量比较差异无统计学意义（P>0.05）。模型组小鼠AT1R和ACE蛋白表达较对照组明显增加（P<0.05），坎地沙坦组AT₁R表达与模型组比较明显降低(P<0.05)，ACE无明显变化（P>0.05）。AT₂R蛋白表达各组间比较差异无统计学意义（P>0.05）。模型组小鼠ACE mRNA表达较对照组明显增加（P<0.05），坎地沙坦组与模型组比较差异无统计学意义（P>0.05）。模型组小鼠AT1R mRNA表达较对照组明显增加（P<0.05），坎地沙坦组较模型组明显降低（P<0.05）。模型组AT2R mRNA较对照组明显降低（P<0.05），坎地沙坦组较模型组明显增加（P<0.05）。各组AGT mRNA表达无明显变化（P>0.05）。结论：在小鼠哮喘发病过程中，RAS活化参与了哮喘的发病过程，并且AT₁R拮抗剂坎地沙坦可以逆转ACE、AT₁R和AT₂R表达的改变。

关键词: 哮喘；肾素-血管紧张素系统；血管紧张素Ⅱ 1型受体拮抗剂

Abstract:

Objective To investigate the relationship between the renin-angiotensin system (RAS)and asthma,and provide the theoretical basis for pathogenesis and treatment of asthma.Methods The mouse asthma models were set up and divided into control group, model group,candesartan low-dose group, and candesartan high-dose group.The serum of mice was collected, the levels of serum angiotensin Ⅱ (AngⅡ) and angiotensin Ⅰ (AngⅠ) were detected;the changes of angiotensinogen (AGT),angiotensin-convertion enzyme (ACE),angiotensin Ⅱ type 1 receptor(AT₁R) and angiotensin Ⅱ type 2 receptor (AT₂R) in the RAS of the mouse lung tissue were delected by Western blotting and RT-PCR.Results The serum Ang Ⅱ level in model group was higher than that in control group(P<0.05),but the level of AngⅡ in candesartan groups had no significant changes compared with model group（P>0.05）.The serum Ang Ⅰlevels in each group had no significant changes（P>0.05）.The expressions of AT1R and ACE protein in model group were markedly increased compared with control group(P<0.05),the AT1R expressions in candesartan groups were significantly decreased compared with model group(P<0.05),but ACE unchanged.There were no significant differences of the expressions of AT2R protein between various groups（P>0.05）.The ACE mRNA expression in model group was increased significantly compared with control group(P<0.05),but there was no significant change in candesartan groups compared with model group（P>0.05）.The AT₁R mRNA expression in model group was significantly increased compared with control group(P<0.05),the AT₁R expressions in candesartan groups were significantly reduced compared with model group(P<0.05).The AT₂R mRNA in model group was significantly decreased compared with model group(P<0.05),the expressions in candesartan groups were significantly increased compared with model group(P<0.05).The expression of AGT mRNA in each group had no significant change.Conclusion In the course of asthma of mice,RAS activation is involved in the pathogenesis of asthma,and the AT₁R blocker can reverse the changes of the expressions of ACE,AT₁R and AT₂R.

Key words: asthma；rennin-angiotensin system； angiotensin Ⅱ type 1 receptor blockers

中图分类号:

孙璐瑶|刘颖|于振香. 哮喘小鼠肺脏组织肾素-血管紧张素系统相关组分表达
及AT₁R拮抗剂对其影响[J]. J4, 2011, 37(6): 1065-1069.

SUN Lu-yao,LIU Ying,YU Zhen-xiang. Expressions of component of renin - angiotensin system in lung tissue of asthmatic mice and effect of AT1 receptor blocker[J]. J4, 2011, 37(6): 1065-1069.

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哮喘小鼠肺脏组织肾素-血管紧张素系统相关组分表达
及AT₁R拮抗剂对其影响

Expressions of component of renin - angiotensin system in lung tissue of asthmatic mice and effect of AT1 receptor blocker

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