关键词: 白血病细胞；细胞凋亡；胡桃醌；MTT法；流式细胞术

Abstract:

Objective
To investigate the inhibitory effect of juglone on the proliferation of leukemic cells and to clarify the mechanism of anti-leukemia of juglone.Methods The leukemic cell lines K562,Jurkat,U937,U266 and NB4 were cultured in vitro and divided into 4 groups as follows: zero group,containing medium,MTT and triple lysate;blank control group,containing a variety of human leukemia cells,drug dissolution media,culture medium,MTT and triple lysate;experiment groups,respectively using different concentrations(final concentration: 0.25,0.50,1.00,2.00,4.00,6.00,8.00,16.00 mg/L)of juglone disposal of various cell lines.The inhibitory effects of juglone of proliferation of leukemia cells were detected by MTT method.The apoptosis of K562 cells after treated with different concentrations of juglone was detected by flow cytometry (FCM).Results Juglone had  strong inhibitory effects on the K562,Jurkat,U937,U266 and NB4 cell lines.Their IC50 (48 h) were 3.87,1.13，4.48,1.87 and 4.67 mg/L,respectively,and the inhibitory effects on Jurkat and U266 cells (P<0.05) were most obvious.The IC50 values  of juglone on other three leukemia cell lines (48 h) were less than 40 mg/L,and the inhibitory effects of juglone on these five cell lines presented dose-and time-dependent manner.FCM analysis showed that juglone could induce the apoptosis of K562 cells;the apoptotic rates of K562 cells were (4.54±0.6) %,(11.5 4±0.6) %,(28.7±0.3) %,(45.0±1.2) % and (76.1±1.4) %,respectively, after treated with  1,2,4,8 and 16 mg?L-1 juglone for 6 h.Conclusion Juglone can inhibit a variety of leukemia cell lines in vitro,and the mechanism may be related to  promoting the apoptosis of K562 cells.

Key words: leukemia cells；apoptosis；juglone；MTT assay；flow cytometry