吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

高剂量维生素E对大鼠氧化应激状态和甲状腺功能的影响

孙景春1,魏倩2,武月婷2,任治兴2,任淑萍2,陈桂秋3,邹运*,杨莹莹*   

  1. 1.吉林大学中日联谊医院检验科,吉林 长春 130033;2. 吉林大学公共卫生学院卫生毒理学教研室,吉林 长春 130021;3. 吉林大学中日联谊医院病理科,吉林 长春 130033
  • 收稿日期:2013-04-07 出版日期:2014-03-28 发布日期:2014-05-28
  • 通讯作者: 陈桂秋 (Tel:0431-89876953,E-mail:guiqiu.chen@126.com) E-mail:guiqiu.chen@126.com
  • 作者简介:孙景春(1966-) ,男,吉林省德惠市人,副教授,医学硕士,主要从事医学检验和临床实验诊断研究。
  • 基金资助:

    吉林省科技厅科技发展计划项目资助课题(200905183)

Effects of high dose of vitamin E on oxidative stress and  thyroid functions of rats

SUN Jing-chun1,WEI Qian2,WU Yue-ting2,REN Zhi-xing2,REN Shu-ping2,CHEN Gui-qiu3,ZOU Yun*,YANG Ying-ying*   

  1. 1. Department of Clinical Laboratory,China-Japan Union Hospital,Jilin University,Changchun 130033,China;2. Department of Hygienic Toxicology,School of Public Health,Jilin University,Changchun 130021,China;3. Department of Pathology,China-Japan Union Hospital,Jilin University, Changchun 130033,China
  • Received:2013-04-07 Online:2014-03-28 Published:2014-05-28

摘要:

目的:检测大鼠体内氧化产物水平和甲状腺相关激素水平,探讨高剂量维生素E(VE)对机体的氧化应激水平及甲状腺功能的影响,为人群VE应用过量有可能产生的问题提供实验参考。方法:30只Wistar大鼠随机分为对照组、溶剂组、低剂量VE组、中剂量VE组和高剂量VE组,每组6只。VE的溶剂为花生油,低、中和高剂量VE组大鼠给药量分别为400、800和1 600 mg/kg。给药30 d后,处死大鼠,检测各组大鼠血清中丙二醛(MDA)浓度、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性、血清中游离三碘甲腺原氨酸(FT3)和血清游离甲状腺素(FT4)水平。结果:中和高剂量VE组大鼠出现毛色变黄且杂乱无光泽,嗜睡、精神萎靡等状态。高剂量VE组大鼠血清MDA浓度明显高于对照组、溶剂组、低和中剂量VE组(P<0.05),中和高剂量VE组大鼠血清SOD活性显著低于对照组、溶剂组、低剂量VE组(P<0.05),中和高剂量VE组大鼠血清GSH-Px活性显著高于对照组、溶剂组、低剂量VE组(P<0.05);与对照组和溶剂组比较,低、中和高剂量VE组大鼠血清FT3和FT4水平显著降低(P<0.05);与对照组和溶剂组比较,中和高剂量VE组大鼠甲状腺滤泡上皮增生明显,细胞核变大且不规则。结论:高剂量VE的摄入可导致大鼠体内发生氧化应激状态,对甲状腺的结构和功能产生影响。

关键词: 维生素E, 氧化应激, 甲状腺功能

Abstract:

Abstract:Objective
To detect the levels of the oxidation products and hormones related to the thyroid,and to discuss the effect of high dose of  vitamin E(VE) on the  level of the oxidative stress and thyroid function of the rats,and to provide the experimental reference for the  questions might be caused by overdose usage of VE in population.Methods 30 Wistar rats were randomly divided into control group(n=6),vehicle group(n=6),low dose of VE group(n=6), medium dose of  VE group(n=6),and high dose of VE group(n=6).The solvent for VE was peanut oil,and the doses of VE for the  rats in low,medium,and high doses of VE  groups  were 400,800,and 1 600 mg/kg, respectively.The  rats were killed 30 d after administration,and  the serum levels of free thyroxine (FT3),free triiodothyronine (FT4),the concertration of malondialdehyde (MDA),and the activities of superoxide dismutase (SOD) and  glutathione peroxidase (GSH-Px) of the  rats in various groups were determined.Results In medium and high doses of VE  groups,the rats’ hair turned yellow and messy and no luster,and they showed sleepiness,mental apathetic state.The concentration  of serum MDA of the rats  in  high dose of VE group  was higher than those in  control,vehicle,low and medium doses of VE  groups (P<0.05);the activity of SOD was significantly increased(P<0.05) and the activity of  GSH-Px was  significantly decreased(P<0.05).Compared with control and vehicle groups,the levels of FT3 and FT4 in low,medium and high doses of  VE groups were significantly decreased (P<0.05).The thyroid follicular epithelial cells of the  rats in medium and high doses of  VE groups  had a higher level of hyperplasia compared with  control and vehicle groups,and  the nucleus became larger and irregular. Conclusion Ingestion of high dose of VE can cause oxidative stress and produce effects on the structure and function of thyroid in the rats.

Key words: vitamin E, oxidative stress, thyroid function

中图分类号: 

  • R151.2