Journal of Jilin University(Medicine Edition) ›› 2020, Vol. 46 ›› Issue (6): 1137-1142.doi: 10.13481/j.1671-587x.20200605

• Research in basic medicine • Previous Articles     Next Articles

Effect of M1 macrophages on immune status in chronic periodontitis model mice

Chen JI1,Wenjuan ZHANG1,Ning GUAN2,Xiuqiu GAO1,Linyuan WANG1()   

  1. 1.Department of Periodontics and Mucosa,Second Affiliated Hospital,Jinzhou Medical University,Jinzhou 121000,China
    2.Key Laboratory of Brain and Spinal Cord Injury,First Affiliated Hospital,Jinzhou Medical University,Jinzhou 121000,China
  • Received:2020-03-25 Online:2020-11-28 Published:2022-08-24
  • Contact: Linyuan WANG E-mail:wanglinyuan2006@163.com

Abstract: Objective

To investigate the effect of M1 macrophages on the immune status of the model mice with porphyromonas gingival (P.gingivalis )-induced chronic periodontitis, and to clarify the role of M1 macrophages in chronic periodontitis.

Methods

Ten 7-week-old female C57BL/6 mice were randomly divided into control group(n=5)and chronic periodontitis group(n=5).The chronic ?periodontitis?models of mice were induced? by? oral? infection? with P.gingivalis. The resorption of alveolar bone of the mice in two groups was estimated by measuring the distance from the cemento-enamel junction(CEJ) to the alveolar bone crest(ABC);HE staining was performed to examine the infiltration of inflammatory cells, attachment loss of connective tissue and resorption of alveolar bone in periodontal tissue of the mice in two groups.? flow cytometry was used to detect the percentages and number of F4/80iNOS M1 macrophages in gingival tissue of the mice in two groups; real-time quantitative PCR method was used to detect the expression levels of iNOS mRNA in gingival tissue of the mice in two groups.

Results

Compared with control group, the distance from CEJ to ABC of the mice in chronic periodontitis group was increased significantly(P<0.01); the gingival junction epithelium attached to the root side of CEJ, and the absorption of ABC became flat, the osteoclasts and bone resorption lacunae of the mice in chronic periodontitis group were found.The number and percentage of M1 macrophages in gingival tissue of the mice in chronic periodontitis group were significantly higher than those in control group(P<0.01).Compared with control group,the expression level of iNOS mRNA in gingival tissue of the mice in chronic periodontitis group was significantly increased(P<0.01).

Conclusion

In the mouse model of chronic periodontitis, the immune response mediated by M1 macrophages is enhanced, and M1 macrophages are involved in the inflammatory response and tissue damage of chronic periodontiti.

Key words: chronic periodontitis, animal models, M1 macrophages, induced nitric oxide synthase

CLC Number: 

  • R781.42