Journal of Jilin University(Medicine Edition) ›› 2022, Vol. 48 ›› Issue (6): 1474-1480.doi: 10.13481/j.1671-587X.20220613
• Research in basic medicine • Previous Articles Next Articles
Lu REN(),Qinxue CAO,Shaoqin YANG
Received:
2022-02-23
Online:
2022-11-28
Published:
2022-12-07
Contact:
Lu REN
E-mail:hls_zhk@163.com
CLC Number:
Lu REN,Qinxue CAO,Shaoqin YANG. Effects of ganoderma lucidum ethanol extract on biological behavior and JAK1/STAT3 signaling pathway of cervical cancer cells[J].Journal of Jilin University(Medicine Edition), 2022, 48(6): 1474-1480.
Tab. 1
Inhibitory rates of proliferation of cervical cancer HeLa cells in varilous groupsafter treated for 24 and 48 h (n=3,x±s,η/%)"
Group | Inhibitory rate | t | P | |
---|---|---|---|---|
(t/h) 24 | 48 | |||
Control | 0 | 0 | - | - |
GLEE Low dose | 7.83±0.60*○ | 15.75±0.75*○ | 11.619 | <0.001 |
Medium dose | 21.69±0.85*△○ | 30.92±1.43*△○ | 7.801 | <0.001 |
High dose | 38.36±1.25*△#○ | 49.53±1.48*△#○ | 8.163 | <0.001 |
Tab. 5
Expression levels of JAK1/STAT3 signaling pathway-related proteins in cervical cancer HeLa cells in various groups"
Group | JAK1 | p-JAK1 | STAT3 | p-STAT3 | SOCS3 | PIAS1 |
---|---|---|---|---|---|---|
Control | 0.79±0.05 | 0.75±0.05 | 0.84±0.07 | 0.83±0.07 | 0.35±0.02 | 0.27±0.03 |
GLEE Low dose | 0.72±0.05 | 0.61±0.05* | 0.86±0.06 | 0.64±0.06* | 0.48±0.04* | 0.44±0.04* |
Medium dose | 0.74±0.04 | 0.43±0.04*△ | 0.85±0.04 | 0.49±0.04*△ | 0.64±0.04*△ | 0.59±0.05*△ |
High dose | 0.76±0.03 | 0.31±0.03*△# | 0.87±0.02 | 0.27±0.02*△# | 0.81±0.06*△# | 0.76±0.06*△# |
1 | HE Y H, SU R J, ZHENG J. Detection of DKK-1 gene methylation in exfoliated cells of cervical squamous cell carcinoma and its relationship with high risk HPV infection[J].Arch Gynecol Obstet,2021,304(3): 743-750. |
2 | BAIK S H, SEO J W, KIM J H, et al. Prognostic value of cervical nodal necrosis observed in preoperative CT and MRI of patients with tongue squamous cell carcinoma and cervical node metastases: a retrospective study[J].AJR Am J Roentgenol,2019,213(2): 437-443. |
3 | JIANG X F, SHI M Q, SUI M, et al. Oleanolic acid inhibits cervical cancer Hela cell proliferation through modulation of the ACSL4 ferroptosis signaling pathway[J]. Biochem Biophys Res Commun, 2021, 545: 81-88. |
4 | LAI P, CAO X, XU Q, et al. Ganoderma lucidum spore ethanol extract attenuates atherosclerosis by regulating lipid metabolism via upregulation of liver Ⅹreceptor alpha[J]. Pharm Biol, 2020, 58(1): 760-770. |
5 | PARK M H, KIM M. Antioxidant and anti-inflammatory activity and cytotoxicity of ethanol extracts from rhynchosia nulubilis cultivated with ganoderma lucidum mycelium[J].Prev Nutr Food Sci,2018,23(4): 326-334. |
6 | LI P, LIU L X, HUANG S, et al. Anti-cancer effects of a neutral triterpene fraction from ganoderma lucidum and its active constituents on SW620 human colorectal cancer cells[J]. Anticancer Agents Med Chem, 2020, 20(2): 237-244. |
7 | FAN Y R, SHENG W W, MENG Y, et al. LncRNA PTENP1 inhibits cervical cancer progression by suppressing miR-106b[J]. Artif Cells Nanomed Biotechnol, 2020, 48(1): 393-407. |
8 | CAO L P, JIN H L, LIANG Q, et al. A new anti-tumor cytotoxic triterpene from ganoderma lucidum[J]. Nat Prod Res, 2022, 36(16): 4125-4131. |
9 | ZHU L P, WU M, LI P, et al. High-pressure supercritical CO2 extracts of ganoderma lucidum fruiting body and their anti-hepatoma effect associated with the ras/raf/MEK/ERK signaling pathway[J]. Front Pharmacol, 2020, 11: 602702. |
10 | LIU Z X, LI Y J, SHE G H, et al. Resveratrol induces cervical cancer HeLa cell apoptosis through the activation and nuclear translocation promotion of FOXO3a[J]. Pharmazie, 2020, 75(6): 250-254. |
11 | 方艳惠, 马彩娟, 张纯丽, 等. miRNA-496通过mTOR影响宫颈癌HeLa细胞的恶性生物学行为及裸鼠移植瘤的生长[J]. 中国肿瘤生物治疗杂志, 2021, 28(11): 1068-1074. |
12 | SOHRETOGLU D, HUANG S L. Ganoderma lucidum polysaccharides as an anti-cancer agent[J]. Anticancer Agents Med Chem, 2018, 18(5): 667-674. |
13 | AHMAD M F, AHMAD F A, KHAN M I, et al. Ganoderma lucidum: a potential source to surmount viral infections through β-glucans immunomodulatory and triterpenoids antiviral properties[J]. Int J Biol Macromol, 2021, 187(9): 769-779. |
14 | 徐凤姣, 曾 琴, 刘宏壁, 等. 灵芝乙醇提取物抗肿瘤活性研究[J]. 中药药理与临床, 2016, 32(6): 127-130. |
15 | ZENG R M, LU X H, LIN J, et al. FOXM1 activates JAK1/STAT3 pathway in human osteoarthritis cartilage cell inflammatory reaction[J]. Exp Biol Med (Maywood), 2021, 246(6): 644-653. |
16 | TAVERNA J A, HUNG C N, DEARMOND D T,et al.Single-cell proteomic profiling identifies combined AXL and JAK1 inhibition as a novel therapeutic strategy for lung cancer[J]. Cancer Res, 2020, 80(7): 1551-1563. |
17 | 罗保卫. miR-19a靶向调控SOCS3激活JAK1-STAT3通路对非小细胞肺癌生物学特性的影响[D]. 衡阳: 南华大学, 2021. |
18 | 李 坤, 王 静, 金春亭, 等. lncRNA GAS5通过miR-26a靶向调控JAK1/STAT3信号通路对子宫内膜样癌细胞凋亡及侵袭的影响[J]. 中国优生与遗传杂志, 2022, 30(3): 357-363. |
19 | 张 琰, 马 娜, 王 芳, 等. 莪术醇抑制JAK1/STAT3信号通路对肺癌细胞增殖、凋亡和侵袭的影响[J]. 临床医学研究与实践, 2021, 6(34): 10-15. |
20 | SIMS N A. The JAK1/STAT3/SOCS3 axis in bone development, physiology, and pathology[J]. Exp Mol Med, 2020, 52(8): 1185-1197. |
[1] | Yujun YUAN,Xiuling YANG,Zhijian HU,Sumei ZHANG. Effects of traditional Chinese medicine indirubin derivative E804 on proliferation, apoptosis and differentiation of lung cancer A549 cells and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2022, 48(5): 1276-1283. |
[2] | Lijing QIN,Bing HAN,Zhongqi LI,Qi SUO,Zhen JIA,Hongli CUI,Weiqiang XU,Fang FANG,Zhicheng WANG. Effects of targeting-silenced ATRX on cell cycle arrest and apoptosis induced by radiation in cervical cancer HeLa cells [J]. Journal of Jilin University(Medicine Edition), 2021, 47(4): 904-910. |
[3] | Dayong XU,Yunpeng LI,Jingmei WEI,Ruyin LIU. Inhibitory effect of baicalin on inflammation in rats with spinal cord injury by regulating macrophage M2 polarization [J]. Journal of Jilin University(Medicine Edition), 2021, 47(1): 158-167. |
[4] | Yinfeng CUI,Mengyu CHAI,Wanshan YANG,Ying PAN. Effect of cardamonin on proliferation of cervical cancer HeLa cells and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2020, 46(6): 1182-1186. |
[5] | WANG Jing, GE Jing, WANG Xu, FANG Guiying, DAI Lili, LIU Jing. Effect of berberine hydrochloride on exosomes of cervical cancer HeLa cells and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2020, 46(04): 779-785. |
[6] | AN Le, YANG Jianzheng, HU Chunmei, YU Qiong, XIAO Han, HAO Shuhong, TANG Yan. Anti-apoptosis effect of polycystin-1 gene on human cervical cancer HeLa cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 776-779. |
[7] | XU LI, ZHANG Wei. Induction of oxaziridine on apoptosis of human cervical cancer HeLa cells and its mechanism [J]. Journal of Jilin University Medicine Edition, 2017, 43(06): 1161-1164. |
[8] | WEN Simin, YU Duo, LYU Xin, WANG Tiejun. Influence of docetaxel combined with cisplatinand single-agent cisplatin concurrent chemoradiotherapy in prognosis of patients with advanced cervical cancer and evaluation on security [J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 1002-1008. |
[9] | DENG Ziliang, WANG Sen, LI Peng, XIE Kuilong, LI Shuxian, ZHOU Shixiong, HE Xiaozhou, CHEN Dong, GUO Hongsheng. Enhancement of IL-37 in chemosensitivity of cervical cancer HeLa cells to cisplatin [J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 862-866. |
[10] | QIANG Xin, HUO Wenyan, YANG Can, FAN Yanan, SHI Caiyun, BAI Haihua. Expressions of p16INK4a protein in cervical lesion tissues of Mongolian patients and their significances [J]. Journal of Jilin University Medicine Edition, 2017, 43(02): 353-355. |
[11] | LIU Qianqian, SUN Lei, BIAN Li, HAN Jinhua, JIA Xiuxiu, YAO Xiaoqing, CHEN Yubing, SONG Xinling. Relationship between cognitive differences in medical behaviors and degrees of anxiety of cervical cancer patients undergoing radiotherapy [J]. Journal of Jilin University Medicine Edition, 2017, 43(01): 115-119. |
[12] | XU Lu, ZENG Linchuan, YU Yang, DOU Minghan, LU Shibing, LI Songyan, XU Ye. Role of Ca2+ in cisplatin-induced autophagy of cervical cancer HeLa cells [J]. Journal of Jilin University Medicine Edition, 2016, 42(06): 1045-1048. |
[13] | ZHOU Jieping, WANG Peng, QIAN Liting, WU Aidong. Dosimetric comparison between IMRT and a single full arc,two full arc VMAT for postoperative cervical cancer patients [J]. Journal of Jilin University Medicine Edition, 2016, 42(01): 120-124. |
[14] | WANG Zhiliang, YU Tenghua, QIN Qin, WU Yutong, ZHANG Wenqian, HUA Yuanyuan, XIONG Zhengai, ZHOU Wei. Influence of irreversible electroporation mediated HPV16 E6 shRNA interference plasmid in proliferation of cervical cancer SiHa cells [J]. Journal of Jilin University Medicine Edition, 2015, 41(06): 1107-1112. |
[15] | ZHANG Wei, WU Yameng, CHEN Siyao, ZHANG Linna, HAN Jingchun, NA Wenting, JIN Ying. Effects of Juglone on proliferation and cell cycle of human cervical cancer SiHa cells [J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 697-700. |
|