Journal of Jilin University(Medicine Edition) ›› 2023, Vol. 49 ›› Issue (2): 440-451.doi: 10.13481/j.1671-587X.20230220
• Research in clinical medicine • Previous Articles Next Articles
Mengmeng CHEN1,Junyu CHEN2,Yan GAO1,Zhengxuan FANG3,Shuhua ZHAO2,Jingying ZHENG2(),Shuxiang LIU1()
Received:
2022-08-07
Online:
2023-03-28
Published:
2023-04-24
Contact:
Jingying ZHENG,Shuxiang LIU
E-mail:zheng_jy@jlu.edu.cn;shuxiang@jlu.edu.cn
CLC Number:
Mengmeng CHEN,Junyu CHEN,Yan GAO,Zhengxuan FANG,Shuhua ZHAO,Jingying ZHENG,Shuxiang LIU. Expression level of gasdermin E in epithelial ovarian cancer tissue and its clinical significance[J].Journal of Jilin University(Medicine Edition), 2023, 49(2): 440-451.
Tab. 1
GSEA gene sets positively correlated with GSDME expression"
ID | ES | NES | P | FDR |
---|---|---|---|---|
REACTOME_GPCR_LIGAND_BINDING | 0.553 | 0.652 | 0.009 | 0.005 |
REACTOME_EXTRACELLULAR_MATRIX_ORGANIZATION | 0.702 | 3.418 | 0.009 | 0.005 |
KEGG_CYTOKINE_CYTOKINE_RECEPTOR_INTERACTION | 0.566 | 2.733 | 0.009 | 0.005 |
REACTOME_ANTI_INFLAMMATORY_RESPONSE_FAVOURING_ LEISHMANIA_PARASITE_INFECTION | 0.630 | 2.963 | 0.009 | 0.005 |
REACTOME_IMMUNOREGULATORY_INTERACTIONS_BETWEEN_A_ LYMPHOID_AND_A_NON_LYMPHOID_CELL | 0.652 | 3.010 | 0.009 | 0.005 |
Tab. 2
GSEA gene sets negatively correlated with GSDME expression"
ID | ES | NES | P | FDR |
---|---|---|---|---|
WP_ELECTRON_TRANSPORT_CHAIN_OXPHOS_SYSTEM_IN_MITOCHONDRIA | -0.748 | -3.115 | 0.009 | 0.005 |
WP_CYTOPLASMIC_RIBOSOMAL_PROTEINS | -0.838 | -3.454 | 0.009 | 0.005 |
REACTOME_RRNA_PROCESSING | -0.672 | -3.145 | 0.009 | 0.005 |
REACTOME_SIGNALING_BY_ROBO_RECEPTORS | -0.555 | -2.611 | 0.009 | 0.005 |
REACTOME_TRANSLATION | -0.628 | -3.094 | 0.009 | 0.005 |
Tab. 3
Results of GO/KEGG enrichment analysis"
Ontology | ID | Description | Gene ratio | BgRatio | Adjusted P | q |
---|---|---|---|---|---|---|
BP | GO:0000395 | mRNA 5'-splice site recognition | 5/359 | 29/18 670 | 0.012 | 0.011 |
BP | GO:0006376 | mRNA splice site selection | 5/359 | 53/18 670 | 0.084 | 0.076 |
BP | GO:0045292 | mRNA cis splicing, via spliceosome | 5/359 | 56/18 670 | 0.096 | 0.087 |
BP | GO:0002455 | humoral immune response mediated by circulating immunoglobulin | 13/359 | 150/18 670 | 0.001 | 0.001 |
BP | GO:0050900 | leukocyte migration | 21/359 | 499/18 670 | 0.028 | 0.025 |
CC | GO:0019814 | immunoglobulin complex | 14/387 | 159/19 717 | 0.001 | 0.001 |
BP | GO:0045765 | regulation of angiogenesis | 18/359 | 383/18 670 | 0.021 | 0.019 |
Tab. 4
Relationship between GSDME protein expression and clinical characteristics of EOC"
Clinical feature | n (η/%) | GSDME | χ2 | P | |
---|---|---|---|---|---|
Positive expression | Negative expression | ||||
Age(year) | |||||
<53 | 17(42.5) | 8 | 9 | ||
≥53 | 23(57.5) | 10 | 13 | 0.051 | 0.822 |
FIGO stage | |||||
Ⅰ-Ⅱ | 19(47.5) | 9 | 10 | ||
Ⅲ-Ⅳ | 21(52.5) | 9 | 12 | 0.082 | 0.775 |
Tumor size | |||||
≤10 cm | 19(47.5) | 8 | 11 | ||
>10 cm | 21(52.5) | 10 | 11 | 0.123 | 0.726 |
EOC type | |||||
Type Ⅰ | 21(52.5) | 10 | 11 | ||
Type Ⅱ | 19(47.5) | 8 | 11 | 0.123 | 0.726 |
Pathological grade | |||||
Low level | 16(40.0) | 11 | 5 | ||
High level | 24(60.0) | 7 | 17 | 6.077 | 0.014 |
Ascites volume | |||||
<500 mL | 21(52.5) | 11 | 10 | ||
≥500 mL | 19(47.5) | 7 | 12 | 0.973 | 0.324 |
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