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LRIG1和 EGFR mRNA在非小细胞肺癌患者外周血中的表达及其临床意义

刘换新1,2,白义凤3,王炜4,郭琳琅2   

  1. 1.武警广东总队医院病理科,广东 广州 510507；2.南方医科大学珠江医院病理科,广东 广州 510282；3.南方医科大学珠江医院肿瘤科,广东 广州 510282；4.广州军区广州总医院病理科,广东 广州 510010
  • 收稿日期:2013-06-24 出版日期:2014-03-28 发布日期:2014-05-28
  • 通讯作者: 郭琳琅(Tel:020-84311872,E-mail:linlang@yahoo.com) E-mail:linlang@yahoo.com
  • 作者简介:刘换新(1969-),男,湖北省广济县人，副主任医师,在读医学博士,主要从事肺癌耐药及转移机制方面的研究。
  • 基金资助:

    国家自然科学基金资助课题（81172241）

Expressions of LRIG1 and EGFR mRNA in peripheral blood of non-small cell lung cancer patients and their clinical significances

LIU Huan-xin1，2，BAI Yi-feng3，WANG Wei4，GUO Lin-lang1   

  1. 1.Department of Pathology,Armed Police Corps Hospital of Guangdong,Guangzhou 510507,China;2.Department of Pathology,Zhujiang Hospital,Southern Medical University，Guangzhou 510282,China;3.Department of Oncology,Zhujiang Hospital,Southern Medical University，Guangzhou 510282,China;4.Department of Pathology,Guangzhou Military Guangzhou General Hospital，Guangzhou 510010,China
  • Received:2013-06-24 Online:2014-03-28 Published:2014-05-28

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摘要：

目的：观察非小细胞肺癌（NSCLC）患者外周血中LRIG1和EGFR mRNA表达水平，并分析其与患者临床病理特征的关系，为NSCLC的治疗提供依据。方法：采用实时荧光定量PCR法分析69例NSCLC患者外周血单个核细胞中LRIG1和EGFR mRNA表达水平，并分析其与患者的肿瘤临床分期、性别、年龄及生存期的关系。结果：与正常人比较，NSCLC患者外周血中LRIG1 mRNA的表达水平降低， EGFR mRNA的表达水平增高，差异具有统计学意义（P<0.001）；LRIG1和EGFR mRNA的表达水平与患者性别、年龄及组织来源无关联(P>0.01 )，与患者肿瘤临床分期及生存期有密切关联(P<0.001)；Cox回归分析，肿瘤临床分期、LRIG1和EGFR mRNA的表达水平可作为独立的预后因子；肿瘤临床分期越晚，患者预后越差（P=0.028），相对风险度（RR）= 1.548,95%可信区间(95% CI)为1.074～2.283；LRIG1 mRNA的表达水平越高，患者预后越好（P= 0.026），RR=0.242,95%可信区间为0.069－0.843；EGFR mRNA表达水平越高，患者预后越差（P=0.027），RR= 3.732，95%CI为1.165～11.956。高表达LRIG1患者的生存时间长于低表达者（χ2 =56.560，P<0.001）；高表达EGFR mRNA患者生存时间短于低表达者（χ2 =51.962，P<0.001）。同一患者血液标本中LRIG1和EGFR 在mRNA表达水平呈负相关关系( r=－0.307,P=0.016)。结论：LRIG1通过负向调节EGFR的表达影响NSCLC患者的肿瘤临床分期和生存时间。LRIG1可能通过调节EGFR的表达影响NSCLC患者的疗效和预后。

关键词: 生存时间, 癌, 非小细胞肺, 实时荧光定量PCR, 表皮生长因子受体, 免疫球蛋白样结构域

Abstract:

Objective
To investigate  the mRNA  expression levels of epidermal growth factor receptor (EGFR) and  leucine-rich repeats and immunoglobulin like domains 1 (LRIG1) in  peripheral blood  from the patients with non-small cell lung cancer（NSCLC）,and to analyze their possible associations with the clinicopathological  parameters of the patients  and provide basis for treatment of NSCLC.Methods The expression levels of EGFR and LRIG1 mRNA were detecteded in peripheral blood of 69 NSCLC patients  by quantitative real-time PCR method.The associations between the gene expression levels and the clinical   stage of tumor, age,gender,and survival time of the patients were analyzed.Results  Compared with normal people,the expression level of LRIGI mRNA in peripheral blood of NSCLC patients was decreased and that of EGFR was increased（P<0.001）.The expression levels of LRIG1 and EGFR mRNA were not associated with gender,age,and histology of the patients(P>0.01 )，but they  were correlated with the  survival time and clinical stage of tumor of the  patients(P<0.001).The Cox regression analysis results indicated that the  clinical   stage of tumor，the expression levels of LRIG1 and EGFR mRNA  were independent prognostic factors for the NSCLC patients.The later the clinical  stage was,the worse the  prognosis was (P= 0.028);the relative risk degree （RR） = 1.548,95% CI：1.074－2.283;the higher the LRIG1 expression was, the better the prognosis of patients was (P= 0.026),RR = 0.242,95%CI：0.069－0.843;the higher the expression of EGFR mRNA was,the worse the prognosis was(P= 0.027),RR = 3.732,95%CI：1.165－11.956.The survival time of the patients with high expression of LRIG1 mRNA  was longer than the patients with lower expression of LRIG1 mRNA (χ2 =56.560,P<0.001);the survival time of the patients with high expression of EGFR mRNA  was shorter than the patients with low expression of EGFR mRNA (χ2 =51.962,P<0.001).The expression levels of LRIG1 and EGFR mRNA in the blood sample of the same patient were negatively related (r=－0.307,P=0.016).Conclusion LRIG1 can affect the clinical stage and survival time of NSCLC patients by negatively regulating the expression of EGFR.LRIG1 may affect the curative effectiveness and prognosis of NSCLC patients by regulating the expression of EGFR.

Key words: cancer,non-small cell lung, quantitative real-time PCR, epidermal growth factor receptor, leucine-rich repeats and immunoglobulin like domains, survival time

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