吉林大学学报(医学版) ›› 2023, Vol. 49 ›› Issue (1): 39-45.doi: 10.13481/j.1671-587X.20230106

• 基础研究 • 上一篇    下一篇

上调Mg2+/Mn2+依赖性蛋白磷酸酶1F表达对鼻咽癌HONE-1细胞增殖和迁移的影响

赵杰1,2,周宁3,刘冬芹4,代娟娟2,王丹丹1,2,武艳1,2()   

  1. 1.滨州医学院附属医院肿瘤科,山东 滨州 256600
    2.滨州医学院附属医院医学研究中心,山东 滨州 256600
    3.滨州医学院附属医院耳鼻咽喉头颈外科,山东 滨州 256600
    4.山东省滨州市人民医院消化内科,山东 滨州 256600
  • 收稿日期:2021-12-26 出版日期:2023-01-28 发布日期:2023-02-03
  • 通讯作者: 武艳 E-mail:wuyan55@126.com
  • 作者简介:赵 杰(1996-),女,山东省聊城市人,在读硕士研究生,主要从事肿瘤发病机制方面的研究。
  • 基金资助:
    国家自然科学基金项目(81903102);山东省科技厅重点研发计划(公益类)项目(2019GSF107099);山东省卫计委医药卫生科技发展计划项目(2017WS154);山东省科技厅自然科学基金项目(ZR2016HB55);滨州医学院科研计划与科研启动基金项目(BY2019KJ04);滨州医学院“临床+X”项目(BY2021LCX23)

Effects of up-regulation of protein phosphatase Mg2+/Mn2+-dependent 1F on proliferation and migration of nasopharyngeal carcinoma HONE-1 cells

Jie ZHAO1,2,Ning ZHOU3,Dongqin LIU4,Juanjuan DAI2,Dandan WANG1,2,Yan WU1,2()   

  1. 1.Department of Oncology,Affiliated Hospital,Binzhou Medical University,Binzhou 256600,China
    2.Medical Research Center,Affiliated Hospital,Binzhou Medical University,Binzhou 256600,China
    3.Department of Otorhinolaryngology Head and Neck Surgery,Affiliated Hospital,Binzhou Medical University,Binzhou 256600,China
    4.Depatment of Gastroenterology,Binzhou People’s Hospital,Shandong Province,Binzhou 256603,China
  • Received:2021-12-26 Online:2023-01-28 Published:2023-02-03
  • Contact: Yan WU E-mail:wuyan55@126.com

摘要:

目的 探讨上调Mg2+/Mn2+依赖性蛋白磷酸酶1F(PPM1F)对鼻咽癌HONE-1细胞增殖、迁移的影响,并阐明其作用机制。 方法 鼻咽癌HONE-1细胞分为pcDNA3.1组(转染pcDNA3.1质粒)和pcDNA3.1-Flag-PPM1F组(转染pcDNA3.1-Flag-PPM1F质粒)。实时荧光定量PCR(RT-qPCR)法和Western blotting法检测2组细胞中PPM1F和E-钙黏蛋白(E-cadherin)mRNA及蛋白表达水平,CCK-8法和克隆形成实验检测2组细胞增殖活性和克隆形成率,细胞划痕实验检测2组细胞划痕愈合率,Transwell实验检测2组细胞中迁移细胞数。 结果 与pcDNA3.1组比较,pcDNA3.1-Flag-PPM1F 组细胞中PPM1F mRNA表达水平明显升高(P<0.01),且PPM1F蛋白表达量明显增加,细胞中E-cadherin mRNA和蛋白表达水平明显升高(P<0.01),细胞增殖活性、克隆形成率和划痕愈合率明显降低(P<0.05或P<0.01),迁移细胞数明显减少(P<0.05)。 结论 上调PPM1F表达能够抑制鼻咽癌HONE-1 细胞增殖和迁移,其机制可能与细胞间的黏附作用有关。

关键词: Mg2+/Mn2+依赖性蛋白磷酸酶1F, 鼻咽肿瘤, 细胞增殖, 细胞迁移

Abstract:

Objective To investigate the effects of up-regulation of protein phosphatase Mg2+/Mn2+-dependent 1F (PPM1F) on the proliferation and migration of nasopharyngeal carcinoma HONE-1 cells, and to clarify their mechanisms. Methods The nasopharyngeal carcinoma HONE-1 cells were divided into pcDNA3.1 group (transfected with pcDNA3.1 plasmid) and pcDNA3.1-Flag-PPM1F group (transfected with pcDNA3.1-Flag-PPM1F plasmid). The expression levels of PPM1F and E-cadherin mRNA and proteins in the cells in two groups were detected by real-time fluorescence quantitative PCR(RT-qPCR) and Western blotting methods; the activities of proliferation and clone formation rates of the cells in two groups were detected by CCK-8 method and clone formation assay; the scratch healing rates of the cells in two groups were detected by cell scratch assay,and the numbers of migation cells in two groups were detected by Transwell experiment. Results Compared with pcDNA3.1 group, the expression level of PPM1F mRNA in the cells in pcDNA3.1-Flag-PPM1F group was significantly increased(P<0.01),the expression amount of PPM1F protein was significantly increased,the expression levels of E-cadherin mRNA and protein in the cells were significantly increased(P<0.01),the proliferation activity,the clone formation rate and the scratch healing rate were significantly decreased (P<0.05 or P<0.01), and the number of migration cells was significantly reduced(P<0.05). Conclusion Up-regulation of PPM1F expression can inhibit the proliferation and migration of nasopharyngeal carcinoma HONE-1 cells, and its mechanism may be related to intercellular adhesion.

Key words: Protein phosphatase Mg2+/Mn2+-dependent 1F, Nasopharyngeal neoplasms, Cell proliferation, Cell migration

中图分类号: 

  • R739.63