Journal of Jilin University(Medicine Edition) ›› 2022, Vol. 48 ›› Issue (6): 1437-1447.doi: 10.13481/j.1671-587X.20220609
• Research in basic medicine • Previous Articles Next Articles
Junxiu LIU1,Jia ZHOU1,Guangfu LYU2,Yuchen WANG1,Xuefeng ZHUANG1,Jiarui ZHAO1,Xiaowei HUANG1(),Ruili LI1()
Received:
2022-03-29
Online:
2022-11-28
Published:
2022-12-07
Contact:
Xiaowei HUANG,Ruili LI
E-mail:15948000740@163.com;377311060@qq.com
CLC Number:
Junxiu LIU,Jia ZHOU,Guangfu LYU,Yuchen WANG,Xuefeng ZHUANG,Jiarui ZHAO,Xiaowei HUANG,Ruili LI. Protective effect of Shenhong Buxue Granule on vascular endothelium of mice with vascular endothelial dysfunction of Qi stagnation and blood stasis type and its mechanism[J].Journal of Jilin University(Medicine Edition), 2022, 48(6): 1437-1447.
Tab. 1
Behavioral performance of mice in various groups"
Group | Grasping forcevalue(m/g) | Number of autonomous activity |
---|---|---|
Normal control | 263.22±76.51 | 53.17±5.21 |
VED | 158.67±37.34* | 35.00±7.96** |
Positive control | 262.15±43.91△△ | 50.83±6.57△△ |
SBG | ||
Low dose | 210.75±32.47△ | 41.83±5.43 |
Middle dose | 229.82±23.01△△ | 44.00±9.63 |
High dose | 255.18±20.17△△ | 48.67±9.74△ |
Tab. 2
Whole blood viscosities of mice in various groups (n=10,x±s,mPa·s)"
Group | Whole blood viscosity | |||
---|---|---|---|---|
10·s-1 | 60·s-1 | 150·s-1 | ||
Normal control | 8.45±1.24 | 5.84±0.82 | 4.44±0.69 | |
VED | 16.23±4.42** | 9.63±2.81* | 7.37±2.62* | |
Positive control | 8.55±2.27△△ | 6.59±0.74△ | 4.57±0.49△ | |
SBG | ||||
Low dose | 10.49±1.67△ | 6.55±0.66△ | 4.60±0.61△ | |
Middle dose | 9.85±1.65△ | 6.26±1.28△ | 4.59±0.50△ | |
High dose | 9.60±0.60△△ | 6.03±1.26△ | 4.55±0.63△ |
Tab. 3
Levels of serum vWF,TM,ICAM-1,and NO of mice in various groups"
Group | vWF [λB/(U·L-1)] | TM [ρB/(pg·L-1)] | ICAM-1 [ρB/(ng·L-1)] | NO [cB/(μmol·L-1)] | |
---|---|---|---|---|---|
Normal control | 255.53±44.10 | 18.11±2.37 | 184.01±8.32 | 26.40±2.09 | |
VED | 486.13±82.92* | 25.83±2.73* | 240.10±1.36** | 16.08±2.09** | |
Positive control | 262.53±45.90△ | 19.63±1.49△ | 197.79±5.50△△ | 24.93±2.09△ | |
SBG | |||||
Low dose | 353.53±47.39 | 23.11±1.24 | 230.48±8.49 | 16.08±4.17 | |
Middle dose | 334.87±94.10 | 22.69±1.51 | 212.53±1.63△△ | 19.03±3.61 | |
High dose | 290.87±47.23△ | 18.58±1.92△ | 211.25±7.85△△ | 21.98±2.09△ |
Tab. 4
Levels of TNF-α and IL-6 in serum and activities of GSH-Px and SOD in thoracic aorta tissue of mice in various groups (n=10,x±s)"
Group | TNF-α [ρB/(ng·L-1)] | IL-6 [ρB/(pg·L-1)] | GSH-Px [λB/(U·mg-1)] | SOD [λB/(U·mg-1)] | |
---|---|---|---|---|---|
Normal control | 626.88±127.88 | 54.62±11.83 | 260.81±12.79 | 52.09±2.43 | |
VED | 1 006.04±96.97* | 100.40±15.85* | 150.35±6.68** | 32.96±2.97** | |
Positive control | 652.92±99.49△ | 57.68±13.95△ | 232.50±10.40△△ | 42.74±2.02△ | |
SBG | |||||
Low dose | 718.54±112.45 | 72.30±8.53 | 182.22±7.84 | 34.87±2.54 | |
Middle dose | 688.33±128.50△ | 70.79±6.66 | 187.06±19.76 | 38.51±1.91 | |
High dose | 656.46±121.80△ | 57.55±13.75△ | 229.52±19.73△△ | 43.54±1.33△ |
Tab. 5
Levels of TNF-α,IL-6,LDH,MDA,NO and activities of GSH-Px and SOD in HUVECs in various groups"
Group | TNF-α [ρB/(ng·L-1)] | IL-6 [ρB/(pg·L-1)] | NO [cB/(μmol·L-1)] | LDH [λB/(U·L-1)] | MDA [mB/(μmol·g-1)] | GSH-PX [λB/(U·mg-1)] | SOD [λB/(U·mg-1)] |
---|---|---|---|---|---|---|---|
Normal control | 66.97±4.35 | 39.65±5.33 | 27.88±6.26 | 202.78±60.48 | 2.13±0.21 | 203.67±11.62 | 40.12±1.88 |
TBHP | 135.27±9.87** | 98.60±6.80** | 14.44±0.23* | 495.37±65.21* | 5.15±0.44** | 115.35±4.44** | 18.62±1.14** |
SBG | |||||||
Low dose | 122.72±6.97 | 82.07±5.38 | 19.03±3.61 | 400.93±68.61 | 3.92±0.22* | 139.93±12.44 | 21.58±1.98 |
Middle dose | 102.14±6.33△ | 72.64±8.17△ | 21.98±2.09△△ | 309.26±47.39△ | 3.39±0.25△△ | 152.74±10.18△△ | 30.52±2.27△△ |
High dose | 78.52±4.90△△ | 53.61±10.78△△ | 23.45±3.61△ | 243.06±80.64△ | 2.87±0.25△△ | 185.77±8.04△△ | 36.04±3.18△△ |
Tab. 6
Survival rates of HUVECs in various groups"
Group | Survival rate |
---|---|
Normal control | |
5% blank serum | 100.00±0.00 |
10% blank serum | 100.00±0.00 |
20% blank serum | 100.00±0.00 |
Low dose of SBG | |
5% drug serum | 95.75±5.55 |
10% drug serum | 98.08±1.15 |
20% drug serum | 93.63±3.32 |
Middle dose of SBG | |
5% drug serum | 99.04±3.86 |
10% drug serum | 95.68±2.56* |
20% drug serum | 86.39±5.78* |
High dose of SBG | |
5% drug serum | 99.84±3.93 |
10% drug serum | 85.17±0.40* |
20% drug serum | 70.54±7.36* |
Tab. 8
Expression levels of TLR4, MyD88,NF-κB p65, and ICAM-1 proteins in HUVECs in various groups"
Group | TLR4 | MyD88 | NF-κB p65 | ICAM-1 |
---|---|---|---|---|
Normal control | 1.00±0.00 | 1.00±0.00 | 1.00±0.00 | 1.00±0.00 |
TBHP | 1.72±0.21** | 1.88±0.31* | 1.97±0.24** | 2.18±0.39* |
SBG | ||||
Low dose | 1.63±0.13 | 1.74±0.29 | 1.81±0.23 | 1.86±0.17 |
Middle dose | 1.55±0.24△ | 1.79±0.37 | 1.99±0.36 | 1.76±0.27 |
High dose | 1.16±0.04△ | 1.24±0.11△ | 1.34±0.08△ | 1.22±0.10△ |
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